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KMID : 0903519960390030195
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1996 Volume.39 No. 3 p.195 ~ p.199
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Regulation of sfs1 gene expression by the cAMP - cAMP receptor protein
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Abstract
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We have cloned several E. coli sfs genes which stimulate mal gene expression with crp^(*1). One the sfs genes (pPVC2) was sequenced and potential CRP binding site is located in the upstream of the putative promoter in the regulatry region. In order to investigate the regulation of the sfs1 gene by the cAMP-CRP complex, we have constructed the sfs-lacZ fusion gene in this research. The overall transcriptional stimulations of sfs1 gene in the presence CAMP were confirmed by β-galactosidase activity and Western blot analysis of sfs1-lacZ fusion gene. Transcriptional regulation by AMP-CRP was also confirmed by Northern blot analysis. End-labelled DNA of the DNA fragment in sfs1 regulatory region were used for gel retardation assay to examine the CRP-DNA complex in the presence of cAMP. Results here indicate that CRP binding site in the regulatory region of sfs1 gene is positive regulator for the expression of sfs1 gene.
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